Bitte versuchen Sie es noch einmal oder kontaktieren Sie den Kundenservice. Bei der Bestätigung Ihrer E-Mailadresse ist ein Fehler aufgetreten. The 30.3 kDa protein shares 30% sequence identity with exonuclease III (ExoIII) of Escherichia coli and 40% sequence identity with the human AP endonuclease Ape1. This 3´→5´ exonuclease will also degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase activity. Exo III is a kind of exonuclease that catalyzes the digestion of duplex DNAs from blunt or recessed 3′-hydroxyl termini (Peng et al., 2012, Zhang et al., 2011, Zhou and Li, 2015). Lokaler Vertrieb Please update your browser to Internet Explorer 11 or above.Um Ihre Daten zu schützen, wird Ihr Benutzerkonto nach 6 falschen Eingaben gesperrt. The gene was amplified from a culture sample and cloned into an expression vector. Please request another reset link.Beim Zurücksetzen des Kennworts ist ein Fehler aufgetreten. We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared with no target recycling. Sie haben ein Promega.com Benutzerkonto angelegt.Überprüfen Sie Ihren Posteingang, um Ihre E-Mailadresse zu bestätigen.Hinweis: Ein Zugriff auf Ihr Benutzerkonto ist bis zur Bestätigung Ihrer E-Mailadresse nicht möglich.Laut unseren Aufzeichnungen wurde die E-Mailadresse bereits registiert.Bei der Erstellung Ihres Benutzerkontos ist ein Fehler aufgetreten. Exonuclease III catalyzes the stepwise removal of mononucleotides from dsDNA starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. Vor-Ort Bevorratung Acids Res. ExoIII catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of double-stranded DNA. The enzyme is not active on single-stranded DNA, and thus 3´-protruding termini are resistant to cleavage. Exonuclease III improves long PCR amplification of UV-irradiated DNA samples. Exonuclease III has four catalytic activities: 3' to 5' exodeoxyribonuclease activity, which is specific for double-stranded DNA; RNase activity; 3' phosphatase activity; AP endonuclease activity (later found to be called endonuclease II). Bitte bestätigen Sie Ihre E-Mailadresse, um Ihr Promega.com Benutzerkonto in vollem Umfang nutzen zu können.There was an issue logging into your account. Software + Firmware Exonuclease VIII, truncated, is able to initiate nucleotide removal from the 5' termini of linear double-stranded DNA in the 5' to 3' direction. When you select your country, you agree that we can place these functional cookies on your device.We've detected that you are using an older version of Internet Explorer.
Promega unternimmt alles um Sie bestmöglich zu beliefern – bitte haben Sie Verständnis für eventuelle Verzögerungen. Im Zuge der aktuellen Geschehnisse und des weltweit ständig steigenden Bedarfs an Reagenzien und Geräten, kann es jedoch aufgrund von Material-Engpässen oder globalen logistischen Einschränken zu Lieferverzögerungen kommen. Exonuclease III (ExoIII) is an enzyme that belongs to the exonuclease family. Since XthA (exonuclease III) has 3′ to 5′ exonuclease activity , we speculated that resection of the DNA ends by this enzyme to produce single-stranded overhangs is crucial for iVEC activity. Erfahren Sie mehr über COVID-19 Produkte und Support Our website uses functional cookies that do not collect any personal information or track your browsing activity. This property is used to produce unidirectional ExoIII activity depends partially on the DNA helical structureExoIII has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities.There are many different exonucleases and many are still to be discovered in bacteria, current studies are being conducted in E. The enzyme is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments. Exonuclease III is a 3´-->5´ exonuclease specific for dsDNA. Support Nucl. Unser Kundenservice und die Technische Beratung helfen Ihnen gerne weiter.